Abstract –This paper presents performance analysis of Multiple-Input Multiple-Output - Orthogonal Frequency Division Multiplexing Access (MIMO-OFDMA) wireless system using orthogonal space time block code (OSTBC) over Rayleigh fading channel. OSTBC is an efficient code system that achieves the maximum of diversity gain in mobile communication systems and it is very useful in reducing the processing complexity of the wireless communication system. Several MIMO-OFDMA OSTBC models based on different number of antenna constellation (2Tx,2Rx) and ( 4Tx,2Rx) have been analyzed. The relationship between Bit Error Rate (BER) and Signal to Noise Ratio (SNR) in Rayleigh fading wireless channel has been studied .The simulation results show the effectiveness and accuracy of the MIMO-OFDMA OSTBC system by decreasing BER for low value of SNR compared with the MIMO-OFDMA system.